Green Fluorescent Protein (GFP) Antibody (FITC) is a Rabbit Polyclonal antibody conjugated to FITC against GFP. Recombinant, monoclonal anti-Green Fluorescent Protein (GFP) single domain antibody (sdAb) fragment: Conjugate: Agarose: Beads ~ 90 µm (cross-linked 4 % agarose beads) Binding capacity: 10 µL slurry bind about 12 µg of recombinant GFP: Elution buffer: SDS sample buffer M glycine pH Storage Buffer: 20% ethanol: Storage Condition. Recombinant, monoclonal anti-Green Fluorescent Protein (GFP) single domain antibody (sdAb) fragment: Conjugate: Magnetic Agarose: Beads ~ 40 µm: Binding capacity: 10 µL slurry bind more than 8 µg of recombinant GFP: Elution buffer: SDS sample buffer M glycine pH Storage Buffer: 20% EtOH: Storage Condition: Upon receipt store at +4. visualized using either fluorochrome-conjugated primary antibodies (direct detection) or a two-step method (indirect detection) involving an unlabeled primary antibody followed by a fluorochrome-conjugated secondary antibody. By combining different fluorochrome-labeled antibodies, multiplex ICC/IF can detect several antigens in the same sample.
Primary and Secondary Antibodies (FL-Immuno/69)
Antigen Species, Others ; Immunogen, GFP from the jellyfish Aequorea victoria N-terminal peptide-KLH conjugates. ; Conjugation, Un-conjugated ; ELISA, Assay-. antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP antibody used in a Sandwich ELISA is well. This antibody reacts with GFP, EBFP, SEBFP, ECFP, SECFP, EGFP, SEGFP, cpSEGFP, cell-specific drug delivery system using apelin-conjugated liposomes. YFP differs from GFP due to a mutation at TY; antibodies raised Synthetic peptide conjugated to KLH, corresponding to N-terminal a.a. of GFP from the. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with a. | Anti-GFP (GOAT) Antibody DyLight™ Conjugated Min X Hu Ms and Rt Serum Proteins.]
Feb 05, · Exposure to light may render poor performance and vial-to-vial variation. Fluorophore-conjugated antibodies also should be stored at 4°C and should never be frozen. For example, BD Biosciences issued a rare recall of fluorophore-conjugated antibody reagents due to high-intensity lighting in its warehouse in A single-domain antibody (sdAb), also known as a nanobody, is an antibody fragment consisting of a single monomeric variable antibody www.wituse.ru a whole antibody, it is able to bind selectively to a specific www.wituse.ru a molecular weight of only 12–15 kDa, single-domain antibodies are much smaller than common antibodies (– kDa) which are composed of . Immunofluorescence and live imaging using a fluorescent fusion protein, such as GFP, Bound antibodies are detected by enzyme-conjugated antibody and the addition of a colorimetric substrate. (C) In immunoblotting, antigens are resolved by gel electrophoresis, transferred to a solid support, e.g. nitrocellulose membrane, and then probed with.
Biotin conjugated polyclonal anti-GFP antibody used in a Sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with a. Host Species, Rabbit ; Immunogen, purified recombinant full-length GFP protein ; Conjugate, Unconjugated ; Form, Lyophilized ; Storage Buffer, lyophilized with PBS. CoraLite®conjugated GFP tag Monoclonal antibody. GFP tag Monoclonal Antibody for FC, IF, WB. Host / Isotype. Mouse / IgG2a. Reactivity. This antibody is conjugated with HRP. Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the. Green fluorescent protein antibody; Images. Western blot - Anti-GFP antibody An undiluted Alexa Fluor ® conjugated Goat anti-chicken polyclonal was used as the secondary antibody. See Abreview. Western blot - Anti-GFP antibody (ab) Image courtesy of an anonymous Abreview. All lanes: Anti-GFP antibody (ab) at 1/ dilution. If using a primary antibody bound to a secondary antibody conjugated to a fluorophore, use only the secondary antibody. Consistent, accurate, and simple-to-use reagents for setting flow cytometry compensation when using green fluorescent protein (GFP). Ease-of-use with a single drop containing both negative and positive beads. Feb 18, · The ability of the AOC to knockdown a gene can be performed by measuring mRNA, Western blotting or incorporating a green fluorescent protein (GFP) into the system. In combination with a fluorophore, the gene silencing activity and trafficking could be correlated together. which allowed the capture of the conjugated antibody with. | Anti-GFP (MOUSE) Monoclonal Antibody DyLight™ Conjugated. Anti-GFP (Green Fluorescent Protein) (Mouse) Texas Red Conjugated Antibody (GWB-D2D74D) | Clone Number: 9F9.F9 | Application: ELISA, WB, IP. Antibodies to detect GFP, ZsGreen1, AcGFP1, or EGFP Monoclonal and polyclonal antibodies to detect green fluorescent proteins. We offer a wide selection of. GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to.
GFP-Tag Rabbit Polyclonal Conjugated Antibody #CT ; Immunogen DescriptionRecombinant GFP expressed in www.wituse.ru ; ConjugatesBiotin AF AF AF AF AF Staining of a tissue section through the midbrain region of an adult mouse for GFP (green) and Tryptophan Hydroxylase-positive neurons (RED). Conjugation to horseradish peroxidase (HRP) simplifies Western blot or ELISA analysis as incubation with secondary antibodies becomes dispensable.
Lane 1: GFP. Load: 50 ng per lane. Primary antibody: GFP antibody Biotin conjugated at , for 60 min at RT. Secondary antibody: Peroxidase streptavidin. A comprehensive review of anti-GFP antibodies including results from Labome to immunoprecipitate GFP-conjugated proteins from A cell lysates []. Product Name, GFP Polyclonal Antibody, ALEXA FLUOR® Conjugated Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology.
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antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP antibody used in a Sandwich ELISA is well. Green fluorescent protein (GFP) from the jellyfish Aequorea Victoria is widely used as a marker of protein expression or targeting in cells or model. Invitrogen Anti-GFP Polyclonal, Catalog # A Tested in Western Blot (WB), Immunocytochemistry (ICC/IF) and Immunohistochemistry (IHC) applications.
FluoTag®-X4 anti-GFP single domain antibody shows a high correlation with the native GFP signal and is superior to conventional GFP antibodies in. Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (www.wituse.ru) and eukaryotic (CHO cells). Green fluorescent protein (GFP) from the jellyfish Aequorea Victoria is widely used as a marker of protein expression or targeting in cells or model.
Anti-GFP Antibody, clone 2B6 Anti-GFP, clone 2B6, Cat. No. MABC, is a mouse monoclonal antibody that detects GFP-conjugated to most proteins and has. Anti-GFP | Green Fluorescence Protein antibodies, peroxidase conjugated, AS15 , recognizing native, recombinant and variants of GFP, anti-rabbit GFP. Green fluorescent protein (GFP) from the jellyfish Aequorea Victoria is widely used as a marker of protein expression or targeting in cells or model.
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